banana dna extraction lab report conclusion
/ca 1.0 All of the above steps are completed in the same manner for the banana DNA extraction except for the adding of water during the smashing of the fruit. ?>. Retrieved from https://phdessay.com/extracting-dna-from-bananas/, Hire skilled expert and get original paper in 3+ hours, Run a free check or have your essay done for you, Didn`t find the right sample? Body identification is a subfield of forensic science wherein investigators need to identify a body. It also controls many of the features that make an organism unique. 0000039985 00000 n <<37B5D6E5CA566B46AF6D8E49138E4E7A>]>> What is DNA extraction and what is its purpose? PhDessay is an educational resource where over 1,000,000 free essays are The type of RNA that helps in mRNA splicing is made by RNA polymerase ________. Lab Report Banana Dna. DNA Extraction from Kiwifruit Student Instructions Introduction DNA is present in the cells of all living organisms. If I mash up a living organism , then I can isolate the DNA . You can use it as an example when writing endobj The book is also an excellent resource for those studying or researching food technology, food science, food engineering, food packaging, applied nutrition, biotechnology, and more. Performance cookies are used to understand and analyze the key performance indexes of the website which helps in delivering a better user experience for the visitors. The order in which the procedure is stated is very important because the cells must first be broken down in order to release the DNA to be seen. Gentlymush the water and banana for 45 seconds. 20 mL DNA extraction buffer (15 g NaCl/ 1 L water) 1 mL liquid dishwashing detergent Ice -cold 95% ethanol or 95% isopropyl alcohol 1/3 banana . Avery and the other scientists discovered that DNA is the nucleic acid that stores and transmits the genetic information from one generation of an organism to the next. you can make your own DNA extraction or animal cells? Do this for 30 to 45 seconds. Hypothesis: 0000003809 00000 n In this biochemistry science project you will make your own DNA extraction kit from household materials and use it to purify DNA from strawberries. Did you know that we have over 70,000 essays on 3,000 topics in our Motivation: Recent technological promotions in the field of genomics have resulted in Following Generation DNA Sequencing Technologies. 166 0 obj <>stream Conclusion Purpose : This lab was conducted in order to show and analyze the way DNA is extracted. DNA Extraction Conclusion - Google Docs Spinach DNA Extraction REE: The results from our experiment have shown that we were not able to spool DNA from eukaryotic cells. But with bananas, we share about 50 percent of our genes, which turns out to be only about 1 percent of our DNA, emails Mike Francis, a Ph. xref 0000006243 00000 n 6T3G]Zq`S;z*H27$21a4a@ aaSn;,8@ U To extract DNA for this activity, it is best to use mushy fruit. By volunteering, or simply sending us feedback on the site. The two strands are held together by hydrogen bonds between adenine and thymine and between guanine and cytosine. For instance, the detergent or soap helps to break down the cells outer membrane, and the salt helps to separate the DNA from other materials in the cell. Lea being the main narrator, sat up on her knees, Inviting the audience In to listen and seemed more comforting and engaged, whereas Phil communicated with the audience with the. For example, scientists can identity genetic cell membranes if the cell wall guards the cell, DNA extraction, a detergent will cause the cell to pop open, or lyse, so that the DNA is released into solution. Do you think the subject Chemistry is necessary or useful on your future career (in Industry, research, academe, engineering, etc.)? The process involves a few general steps, including mashing, filtration, precipitation, and extraction. There are four types of nucleotides which vary depending on the four nitrogenous bases of the molecule. % We are able to properly and successfully extract DNA from banana using cell disruption and separation techniques. My hypothesis was correct for strawberries producing the most DNA followed by kiwis and than bananas last. >> Result Photo 1: Test tubes containing solution S and SS CONCLUSION. your own essay or use it as a source, but you need 6. Materials for each group of students: /Filter /DCTDecode The salt contributes positively charged atoms that neutralise the normal negative charge of DNA. Why or why not? In this activity each material plays a specific role in helping to extract the DNA from the cells. The Mendelian Revolution: The Emergence of Hereditarian Concepts in Modern Science and Society. The soap breaks apart the cell membrane and the cell parts that hold the DNA. All living things including humans, plants (bananas!) 6 : Group Bs Results Tan 1 1. These cookies will be stored in your browser only with your consent. The filtration step (pouring the mixture through the strainer) allows for the collection of the DNA and other cellular substances. Add the dishwashing soap into the bag and gentlymix, trying not to make it foam up. DNA stands for deoxyribonucleic acid, which is a nucleic acid that contains the sugar deoxyribose. The mixture was divided into two 50ml conical flasks (20ml each). and animals have DNA (deoxyribonucleic acid). ASU - Ask A Biologist. So when seeing it without a high-poweredmicroscopewhat does DNA look like? /Height 155 After the alcohol layer is set up, wait for eight minutes. Variants of genes, called alleles, are responsible for differences in hair color, eye color, and earlobe shape. Complementarity serves as the basis for both the replication of DNA and for the transcription of DNA into RNA. Then a plastic pipette was filled with the filtrate two times and added to the alcohol. In this activity, you will extract DNA from banana, strawberry and kiwi fruits. * To investigate the effect of temperature on DNA extraction from bananas. During the DNA extraction, the soap pulls apart the fats (lipids) and proteins that make up the membranes surrounding the cell and nucleus. It is amazing how important things are being discovered by persons who spend most of their lives dedicated to research. You are looking at DNA! Essay. Banana bunchy top of your problem is a few minutes without seeds and reseeding of cookies before placing the parts of genomic distribution, but any spills. extract dna from a banana thoughtco, lab report 6 bacterial dna extraction ibg1022018, maya g biology human dna extraction lab conclusion, dna lab report 1345 words bartleby, doc dna extraction of strawberry and banana jefferson, find the dna in a banana scientific american, conclusion dna extraction conclusion the purpose of this, dna . Pour the mix into the filter and let it sit until all of the liquid drips down into the cup. Then the salt and shampoo had to be dissolved into the mixture by stirring slowly by avoiding foaming. Explore how the human body functions as one unit in The solution had to be blended for 15 to 20 seconds, until it became a dense mixture. /Title () Remove and discard the used coffee filter. In the cytoplasm, the information in RNA is translated into proteins, which serve as the end products of most all genes. 1 0 obj Extracting DNA from a Banana and Other Fruits Introduction All living things, bananas and people included, pass on information from one generation to the next using the same basic material, DNA. 0000040917 00000 n ->!W[wde@ =:| 8 :>ijzKO g9 0000002514 00000 n Write reflection paper about this question. The process of transferring information from DNA to RNA is called transcription. The bananas were mashed for about 5 minutes. Once these membranes are broken apart, the DNA is released from the cell. The bag was closed with minimum content of air. The banana mixtures were cooled in the ice chest containing ice for 2 minutes. %%EOF The total number of DNA for each fruit had a big variation between strawberries compared to kiwis and bananas with a difference of 5 and 6. In eukaryotic organisms, RNA most ofther carries the genetic information out of the nucleus, where chromosomes reside into the cytoplasm of the cell. ?>, Order original essay sample specially for your assignment needs, https://phdessay.com/extracting-dna-from-bananas/, A Passion for DNA: Genes, Genomes, and Society, A role for transportin in the nuclear import of Adenovirus core proteins and dna, get custom The sa$t e"ab$es the DN stra"%s to, The DN prec#p#tates out o! However, you may visit "Cookie Settings" to provide a controlled consent. We do in fact share about 50% of our genes with plants including bananas. Bananas have 44.1% of genetic makeup in common with humans. Humans share 50% of our DNA with a banana.. Cells reproduce in part by passing deoxyribonucleic acid (DNA) from parent cells to offspring cells. /SM 0.02 0000004908 00000 n DNA is a component of living and once-living things. It holds the instructions necessary for the organism to grow and function, and is passed on from generation to generation through heredity. Let us help you get a good grade on your paper. endobj Close the bag and very gently squeeze and move the saltwater and banana mush together. These instructions are in segments of DNA called genes. A. potential of crossing over in meiosis I B. We are able to observe the extraction of genomic DNA from plant cells. Retrieved March 2, 2023 from https://askabiologist.asu.edu/activities/banana-dna, Melissa Wilson Sayres. Type your requirements and I'll connect Materials: Extracting DNA from Bananas student handout 1 large banana 3/4 cups distilled . These comprise the genetic alphabet which in various combinations, will specify the components of proteins. Sometimes it is hard to do all the work on your own. w !1AQaq"2B #3Rbr All of the above steps were completed in the same manner for the banana DNA extraction except DNA and small cell debris to filter through. Step 2: In a separate bowl, mix the washing up liquid, salt and tap water. Which is correct poinsettia or poinsettia? Materials: Half a peeled banana distilled water plastic sealable bag or beaker plastic cup shampoo or dishwashing liquid table salt In a separate five-ounce cup, a solution consisting of one teaspoon of shampoo and two pinches of salt was made. $4%&'()*56789:CDEFGHIJSTUVWXYZcdefghijstuvwxyz ? Add the dishwashing soap into the bag and gently mix the contents. JFIF d d C Dietary Reference Intakes for Vitamin C . <>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/Annots[ 14 0 R 15 0 R 16 0 R 17 0 R 23 0 R] /MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> ", American Psychological Association. 0000004435 00000 n Once extracted, DNA could be observed and inferences made about the nature of DNA, and then compare the differences between two DNA's amount. endobj re$ease% !rom the ce$$. This website uses cookies to improve your experience while you navigate through the website. you to an academic expert within 3 minutes. This process was repeated for 4 times. Save time and let our verified experts help you. Use the wooden stirrer to start poking the cloudy stuff in the alcohol layer. This lab was conducted in order to show and analyze the way DNA is extracted. f MATERIALS Fresh Bananas Droppers Water 70% rubbing alcohol 3 Plastic Bag (Ziplock) Salt Liquid Dish Soap Mixing bowl (Medium size) Measuring spoon Measuring cup 3 Beaker (Big and LECTURER'S NAME: DR. LAM NYEE FAN. We use cookies on our website to give you the most relevant experience by remembering your preferences and repeat visits. The coffee filter was specifically placed in the cup so that it did not touch the bottom. It does not store any personal data. Course Hero is not sponsored or endorsed by any college or university. These cookies ensure basic functionalities and security features of the website, anonymously. (B) Banana Extraction 1. Isopropyl rubbing alcohol allows me to see the DNA because when molecules are insoluble the clump together and become visible so the alcohol makes the DNA insoluble making it become visible. With Extracting DNA from Bananas In the Lab: Extracting DNA from Bananas, DNA was removed from bananas that had been blended with water in order to examine how DNA is seen from the naked eye. essay, Genome Patterns of Common DNA Variations in Three Human Populations, Strawberry DNA Extraction Lab Formal Write Up, Next Generation DNA Sequencing Technologies Biology Essay, Write cookie policy. Johns Hopkins University Press. support@phdessay.com. The cookie is used to store the user consent for the cookies in the category "Analytics". 4 0 obj //= $post_title The bananas were mushed carefully to avoid the bag from breaking. 3 For what purposes is DNA extraction done give at least 3 purposes for which you may need to extract DNA? We manage to learn basic DNA extraction processes. 5 0 obj 3g PROCEDURE (A) Extraction solution recipe: 4ml of shampoo was mixed with 36ml of distilled water. What do we have in common with the banana plant? Even though the procedures vary considerably, they involve three main steps: lysis of cell wall, cell membrane and nuclear membrane, isolation of DNA from other cell inclusions, and purification of the extracted DNA. In addition, there are four kinds of nitrogenous bases in DNA. 0000008293 00000 n This DNA extraction is simple and works every time but the DNA is not pure. Look over some of the links provided and develop a hypothesis and design an experiment that will allow you to perform your own DNA extraction. ma)e up the membra"es surrou"%#"g the ce$$ a"% "uc$eus. 0000003969 00000 n Of those 60 percent, the proteins encoded by them are roughly 40 percent identical when we compare the amino acid sequence of the human protein to its equivalent in the banana, Brody adds. You may see some bubbles and cloudy material moving around in the alcohol. This is the DNA pieces clumping together. Begin typing your search term above and press enter to search. 135 0 obj <> endobj Materials: Extracting DNA from Bananas student handout 1 large banana 3/4 cups distilled . The nanodrop system revealed that at an absorption of 260 wavelengths, the DNA measured 7.49 using a 374.66 ng/ul sample. Descr#be the appeara"ce o! A water bath was prepared. /SMask /None>> How do I choose between my boyfriend and my best friend? The test tubes were then observed. 0000044903 00000 n xnFab5\8CA _4E$`i,yI:=x-Yd.5g:#6}^={s/0lH*|?x'}=]4}vb ~M4q'(OV%l`! Mashing the banana exposes a greater surface area from which to extract the DNA. You share 50 percent of your DNA with each of your parents. The banana DNA was easy to see as it was a light pink/clear and it was chunky and had thick pieces when picked up with the skewer but it appeared shredded in the liquid. We succesfully investigated the effect of temperature on DNA extraction from bananas. 0000006862 00000 n Each strand is made up of a chain of nucleotides. Banana DNA Extraction Lab Name: _____ Objectives: Describe where DNA is located in a plant cell. The remaining two bases, cytosine and thymine, are known as pyrimidines. 0000002648 00000 n If the steps were altered then the yield or the amount of DNA per banana could have been very small because the DNA would get trapped inside the cells or bound to lipids. <>>> Then the DNA can be precipitated out of the solution by adding alcohol. Order custom essay Extracting Dna from Bananas DNA is made up of a series of monomers called nucleotides. endstream endobj 165 0 obj <>/Size 135/Type/XRef>>stream /Width 625 the DN +co$or' texture', The soap so$ut#o" co"ta#"s so%#um $aure$ su$!ate' wh#ch, the soap pu$$s apart the !ats +$#p#%s a"% prote#"s that. &t #s, #"cre%#b$y sma$$' but we ca" see #t by extract#"g DN a"%, %#st#$$e% water #" the p$ast#c bag' sea$ the bag a"%, so$ut#o" s$ow$y !or about a m#"ute u"t#$ the shampoo, the empty cup. 19 April, 2016. https://askabiologist.asu.edu/activities/banana-dna, Melissa Wilson Sayres. /Creator ( w k h t m l t o p d f 0 . Aim: To extract DNA from a banana using simple household items Hypothesis: DNA is found in the cells of every living organism. can use them for free to gain inspiration and new creative ideas for their writing An academic unit ofThe College of Liberal Arts and Sciences, You may need to edit author's name to meet the style formats, which are in most cases "Last name, First name. As part of the chromosomes, the information contained in genes can be transmitted faithfully by parents through gametes to their offspring. May 2nd, 2018 - The banana DNA will begin to rise and precipitate out of solution Documents Similar To Lab Report 4 DNA Isolation Bio DNA Extraction Lab Report Lab Reports ScienceHK April 24th, 2018 - To learn the simple method of DNA extraction Take a picture which will be included in your lab report Who says lab reports can t be fun endobj Kiwis and bananas both together produced the same amount of strawberries alone. 20 milliliters (four teaspoons) of distilled water was then added to the mixture. * Peter J. Bowler (1989). Some additional protocols for DNA extractions: Sex Influenced Female Dominant Inheritance, beakers 100ml and 2x250ml (or jars that same size), liquid dishwashing detergent (clear solution works better than cloudy), Make a 10% solution of dishwashing detergent in one of your 250ml beakers (make enough to fill the mortar half way), Add the 10% detergent solution to cover the fruit, Grind the banana in the soap solution until there are no lumps (this is the hardest part, have the students take turns), Fasten a coffee filter to the 250 ml beaker with a rubber band so there is room for the fruit to be poured into the filter, Pour the fruit and soap mixture into the filter and place in a cold place to filter, Clean up your lab area while you are waiting for the filtering to complete, Remove the filter and throw away the contents, Pour enough of the liquid into a test tube to fill it 2 cm high, Add twice the volume of 95% ethanol (try to layer the alcohol on top by gently pouring it down the side of the test tube), The DNA will precipitate in the boundary between the alcohol and the banana mixture as a cloudy mass. 0000001999 00000 n You also have the option to opt-out of these cookies. Although most mutants are either impersonal or harmful they are besides the natural stuff for development. This complementarity between adenine and cytosine nitrogenuos base pairs and between guanine and cytosine nitrogenuos base pase pairs, attracted to one another by hydrogen bonds, is critical to genetic function. 9 How does detergent help to break down DNA? number: 206095338, E-mail us: Necessary cookies are absolutely essential for the website to function properly. The liquid soap is added to help break down cell membranes to release the DNA. Scientists, teachers, writers, illustrators, and translators are all important to the program. ASU - Ask A Biologist, Web. 8. 1. DNA is a component of living and once-living things. The banana DNA was circular and was very separated into many smaller pieces. every $#v#"g orga"#sm. Lastly, All Papers Are For Research And Reference Purposes Only. 135 32 Ef+\:IZy}r(| F @q!4}g' U-CBb8sxurgg$t@AumUg Sx}Pa=cWyyh>WikxqB(;I&dCf&7CgCr(zMQl`PL BXIfBk. Approximately 3ml of banana solution were dispenced into each test tube. o/ H|9 O7ze~.p -s'NK SNlg7WO dq=}i43 Water is a molecule of two hydrogen atoms and one oxygen atom (H2O)more. 0000003225 00000 n You may understand that mashing a banana can break cells apart and help break apart cell walls, but why was all that other stuff added? The white DNA could be seen precipitating out of the alcohol layer. 0000000016 00000 n DNA extraction is a method to purify DNA by using physical and/or chemical methods from a sample separating DNA from cell membranes, proteins, and other cellular components. 5. Bananas and strawberries are great choices. 0000000936 00000 n The test tubes were carefully handled to avoid shaking. <> Each nucleotide has three parts: a deoxyribose molecule, a phosphate group, and a nitrogenous base. Some of the protein is still present. How does detergent help to break down DNA? Step 8: Pour the drained liquid into a glass. DNA can be extracted and observed. } !1AQa"q2#BR$3br 1 1 . % Breaking cells open to release the DNA. DNA Extraction Lab Begin by watching the Video of a Strawberry DNA extraction, also available in the module, and think about how DNA extraction is possible. 9>U0c4S7FK9>>Ns^|t_g7]}|qwwQvvpaZEU2pOUAK2wb)m-]v| 0000044671 00000 n Studying DNA is one way scientists learn about what is necessary for life. Because of their size, it is impossible to see a single DNA molecule with the naked eye. cite it. xb``a``f Y800* @1*?./j Extraction solution 'S' was added into ziplock 'Extraction 1' and extraxtion solution 'SS' into ziplock bad . Free Samples and Examples of Essays, Homeworks and any Papers, Filed Under: Essays Tagged With: Genetics. Mush the banana in the resealable bag for about a minute until all the lumps are gone and it almost looks like pudding. And how did we get inside the cells and get the DNA to stick together? Advertisement cookies are used to provide visitors with relevant ads and marketing campaigns. endobj endobj Two of the nitrogenous bases, adenine and guanine, belong to a group of compounds known as purines. About 60 percent of our genes have a recognizable counterpart in the banana genome! * Elof Carlson (The Unfit), Mendels Legacy: The Origin of Classical Genetics, Cold Spring Harbour Laboratory Press, USA * Benjamin Cummings(2005), iGenetics: A Mendelian Approach, Pearson; University of Chicago, USA. You don't want the mixture to become too foamy. /BitsPerComponent 8 What properties do bases have? endobj However, it is nice because the students get to see and hold in their hand the substance they have been studying that seems so abstract and hard to believe it even exists. We even have genes in common with the banana plant! For more info, see, https://askabiologist.asu.edu/activities/banana-dna, Public Service and Gene: a region of DNA that instructs the cell on how to build protein(s). Thus, DNA is present in all living organisms. Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. You must cite our web site as your source. Then the DNA can be precipitated, or come out of the solution, by adding alcohol. All living things, bananas and people included, pass on information from one generation to the next using the same basic material, DNA. While the banana solution was being mixed, a number two cone coffee filter was laced inside a second five-ounce cup. It was established in 1953 by James Watson and Francis Crick that the two strands of their proposed double helis are exact complements of one another, such that the rings of the ladder always consists of either A=T, or G=C base pairs.